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Fig. 4 | BMC Neuroscience

Fig. 4

From: Selenium suppresses glutamate-induced cell death and prevents mitochondrial morphological dynamic alterations in hippocampal HT22 neuronal cells

Fig. 4

Immunocytochemistry and protein blotting of Apaf-1. Apaf-1 (green color) was detected using antibody after 24 h of glutamate or glutamate plus selenite treatments. DAPI (blue color) was used to label the nuclei. Western blot was performed using cytosolic fraction. Relative band intensity was measured and presented in the bar graph. Β-tubulin served as internal protein loading control. Glutamate incubation increased the Apaf-1 protein band intensity to 51.8 ± 1.4 and concurrent treatment with selenite reduced the value to 32.7 ± 1.4. F(2, 29) = 97.1. *p < 0.05 and **p < 0.01 versus control; # p < 0.05 versus Glutamate by ANOVA with post hoc Tukey’s test. N = 10 in each group

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