Fig. 5

Exogenously expressed KCC2a and KCC2b mediate efficient furosemide-sensitive chloride extrusion in Neuro-2a cells. a Mean values for the Cl⁻ extrusion rate in the presence and absence of furosemide obtained in the individual experiments similar to those shown in Fig. 4. The rate of chloride extrusion is significantly decreased by furosemide (500 μM), a known blocker of K–Cl transport, in KCC2a (n = 55) and KCC2b (n = 40) transfected cells. (*** p < 0.005; ns, nonsignificant; two-sided Student’s t-test; Error bars represent SEM). b Neuro-2a cells expressing KCC2a (n = 55) and KCC2b (n = 40) constructs demonstrate a significantly higher furosemide-sensitive Cl⁻ extrusion rate compared to the mock transfected cells (n = 20). (*p < 0.05; ***p < 0.005; ns nonsignificant; Kruskal–Wallis nonparametric ANOVA). Mean values and corresponding SEM are shown