Fig. 3

Glycine in combination with high K⁺ provides an efficient chloride loading into Neuro-2a cells exogenously expressing glycine receptors. a In the absence of high K⁺ in the extracellular solution (ECS), application of glycine to Neuro-2a cells expressing Gly receptors results only in a minor increase in the intracellular chloride concentration. In non-depolarized Neuro-2a cells, chloride loading is inefficient even in cells with relatively low [Cl⁻]_i and may be completely blocked in cells with high [Cl⁻]_i levels. Mean values and corresponding SEM are shown for n = 13 recorded cells. b Application of glycine in combination with high K⁺ strongly increases the intracellular chloride concentration. Note that in the depolarized conditions opening of the Cl-permeable channels upon glycine application always results in a robust Cl⁻ accumulation. Mean values and corresponding SEM are shown for n = 20 recorded cells. c Application of high K⁺ along increases the intracellular chloride concentration at the rate about twice slower compared to the co-application of glycine and high K⁺. Mean values and corresponding SEM are shown for n = 14 recorded cells