Fig. 3

Laterality of bioluminescence signals from visual cortex after monocular deprivation (MD) by suturing and reopening surgery. a Experimental schedule for monocular deprivation and reopening and BLI. The time points of BLI are indicated with black triangles. b The obtained bioluminescence images (pseudocolored, 0–15,000 counts) before eye suture (Control on P21), 1 day after MD (MD1 on P22), 7 days after MD (MD7 on P28), immediately after reopening (RO on P29), and 1 day after RO (RO1). Mice were exposed to an intense light for 3 h before each imaging time point. The locations of regions of interest (ROIs) are enclosed in dotted circles in the control image, and the obtained bioluminescence signal intensities are used for the calculation of lateralization index (LI) shown in (c). Scale bar 10 mm. c Plots showing the LI under each condition shown in (a) (n = 6). LI was calculated using the formula (L − R)/(L + R), where L and R are the bioluminescence signal intensities of ROI in the regions contralateral (left) and ipsilateral (right) to the sutured eye, respectively. LI was nearly zero under the control condition (−0.009 ± 0.004), decreased below zero under MD conditions (MD1, −0.213 ± 0.012; MD7, −0.134 ± 0.015), and increased immediately at RO (RO, +0.070 ± 0.016), and finally returned close to the baseline at RO1 (RO1, −0.011 ± 0.016). The data represent mean ± SD. *p < 0.05; two-tailed Student’s t test