Fig. 2
From: Application of hairless mouse strain to bioluminescence imaging of Arc expression in mouse brain
Noninvasive BLI using Arc-Luc Tg HL mouse. a Bright field (upper panels) and bioluminescence signal images (lower panels, pseudocolored, 0–7500 counts) of an individual Arc-Luc Tg HL mouse from 4 to 8 weeks of age. Bioluminescence signals in the Arc-Luc Tg HL mouse were obtained after luciferin injection (i.p., 200 mg/kg BW) under anaesthesia induced with isoflurane. Scale bar 20 mm. b Western blot analysis of proteins in the cerebral cortex was performed using anti-Luc (upper panel), anti-Arc (middle panel), and anti-actin (lower panel) antibodies (n = 3). Location of protein size markers are indicated on the right side. The optical density of each band was quantified using NIH ImageJ. The Luc and Arc protein expression levels normalized to actin protein expression level at 4 and 8 weeks of age are shown in the bar graph (right panel). The data represent mean ± SD. *p < 0.05; two-tailed Student’s t test