Fig. 6

Characterization of individual ALS plasma samples with the different scFvs. Human plasma samples from 4 sporadic ALS cases, 4 c9orf72 ALS cases and 3 healthy control cases were assayed for the presence of TDP-43 variants. Results of the 9 most reactive scFvs are shown: a ALS-TDP3, b ALS-TDP5, c ALS-TDP6, d ALS-TDP7, e ALS-TDP10, f ALS-TDP11, g ALS-TDP15, h ALS-TDP17 and i AD-TDP2. A biotinylated TDP-43 phage capture ELISA system was utilized to enhance our detection capabilities. On average, all the scFvs showed significantly greater reactivity with the sporadic ALS cases relative to the controls. ScFvs ALS-TDP3, ALS-TDP6, ALS-TDP7, ALS-TDP17 and AD-TDP2 also showed statistical differences between the sporadic ALS cases and the c9orf72 ALS samples. In addition, ALS-TDP10 could significantly distinguish between the c9orf72 ALS and control groups. Statistical significance is based on one-way ANOVA with LSD post hoc analysis at p < 0.05. Signal intensities are based on the number of standard deviations each sample is from the controls. This is calculated by subtracting the mean of the controls from each sample and then dividing the result by the SD of the controls