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Fig. 5 | BMC Neuroscience

Fig. 5

From: TDP-43 protein variants as biomarkers in amyotrophic lateral sclerosis

Fig. 5

Characterization of individual ALS brain tissue samples with the different scFvs. Assessment of the binding specificities of the scFvs with brain tissue (motor cortex) from 5 individual ALS cases and 5 controls (as opposed to with pooled samples used in past studies). Results with the 12 most reactive scFvs are shown: a ALS-TDP4, b ALS-TDP5, c ALS-TDP6, d ALS-TDP9, e ALS-TDP10, f ALS-TDP11, g ALS-TDP13, h ALS-TDP14, i ALS-TDP15, j ALS-TDP20, k AD-TDP1 and l AD-TDP3. Here we utilized a biotinylated TDP-43 phage capture ELISA system to enhance our detection capabilities. On average, all 12 scFvs showed significantly greater reactivity with the 5 ALS brain tissue samples compared to the controls. Statistical significance is based on one-way ANOVA with LSD post hoc analysis at p < 0.05. Signal intensities are based on the number of standard deviations each sample is from the controls. This is calculated by subtracting the mean of the controls from each sample and then dividing the result by the SD of the controls

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