Fig. 8

Effects of PJ34 on neurosphere formation and cell viability of Trp53 ^+/− and Trp53 ^−/− NSPCs. a Western blot analysis demonstrated that the amounts of p53 protein and phosphorylated p53 at Ser18 were increased by PJ34 in Trp53 ^+/− NSPCs as well as wild-type NSPCs. Automodification of PARP-1 was detectable irrespective of Trp53 genotype. b Neurospheres were detectable in wild-type NSPCs after a 2-day incubation in the absence of PJ34, but were scarcely detectable with 10 or 20 μM PJ34. In contrast, neurospheres were still detectable with 10 μM PJ34 in Trp53 ^+/− NSPCs, and with 10 or 20 μM PJ34 in Trp53 ^−/− NSPCs. c The increase of MTS-reduction activity of wild-type NSPCs was suppressed by 10 or 20 μM PJ34. No suppressive effect of PJ34 was observed in Trp53 ^−/− NSPCs. Data represent the mean value ± SEM (n = 3). *p < 0.05 and ^† p < 0.01 by one-way ANOVA followed by Tukey’s post hoc test. Scale bars in (b), 50μm