Fig. 6

Effects of PARP-1 or PARP-2 knockdown on the p53 signaling pathway in NSPCs. Quantitative mRNA analyses after silencing Parp1 or Parp2 by shRNA-expressing lentivirus were performed for Parp1 and Parp2 (a) and the genes in the p53 signaling pathway (b). Genes in the p53 signaling pathway were upregulated by the suppression of Parp1 expression, but not by Parp2 suppression. Data shown in (a, b) are expressed as the ratio of the mean value of the sh Control group. Data represent the mean value ± SEM (n = 3). ^† p < 0.01, ^‡ p < 0.005, and ^§ p < 0.001 by comparison against control using Student’s t test