Fig. 4

Induction of apoptosis in NSPCs by PARP inhibitors. a NSPCs were stained with Hoechst 33258 after a 24-h incubation with the PARP inhibitors. Cells with condensed chromatin, which is indicative of apoptosis, were detectable after the addition of PJ34, DHIQ, or 3AB. The ratio of apoptotic cells increased in a dose-dependent manner. b The rate of Annexin-V-positive cells after the addition of 20 μM PJ34 was analyzed by the use of flow cytometry staining with APC-conjugated Annexin-V and 7-AAD. Data represent the mean value ± SEM (n = 6). ^‡ p < 0.005 by comparison against control using Student’s t test. Scale bars in (a), 20μm