Table 1 Real-time PCR primers
Gene | GenBank | Primer sequence | Product length (bp) |
|---|---|---|---|
TNFα | MN_012675 | F tgacccccattactctgacc R ttcagcgtctcgtgtgtttc | 152 |
INFϒ | MN_138880 | F agcatggatgctatggaagg R ctgatggcctggttgtcttt | 146 |
IL4 | MN_201270 | F gaaaaagggactccatgcac R tcttcaagcacggaggtaca | 145 |
TGFβ3 | NM_013174.1 | F cccaacccccagctccaagcg R cagccactctgcggtggctc | 132 |
BDNF | MN_001270630 | F tggcctaacaatgtttgcag R cagctccacttagcctccac | 114 |
IGF1 | MN_001082477 | F cttaggggctagcctcaggt R gttccgatgttttgcaggtt | 158 |
GAP43 | NM_017195.3 | F ctaaggaaagtgcccgacag R gcaggagagacagggttcag | 117 |
β-ACTIN | NM_007393 | F agggccaaccgtgaaaag R gtggtacgaccagaggcatac | 110 |
HPRT | NM_012583.2 | F aagcttgctggtgaaaagga R caaagcctaaaagacagcgg | 192 |