Fig. 5
Gen reduced cytochrome c and Smac release induced by Aβ25–35 in PC12 cells. PC12 cells were pretreated with or without Gen at concentrations of 25 μM for 2 h followed by exposure to 20 μM Aβ25–35 for 24 h. SP600125 (100 nM) was added to cultures 1 h prior to Aβ25–35. a Cytochrome c levels were determined by Western blot analysis with antibody to cytochrome c. b Smac levels were determined by Western blot analysis with antibody to Smac. c Quantitated results of Cytochrome c are presented relative to control. d Quantitated results of Smac are presented relative to control. Densitometric analysis of Western blot obtained from three separate experiments, and data are expressed as mean ± SD, n = 3. *p < 0.05 compared to control; #p < 0.05 compared to Aβ alone