Fig. 4

Brain injury evaluations in the conscious and the anesthetic groups. a, b NSS and infarct volume from TTC staining in the conscious group (n = 11) 24 h post occlusion showed significantly worse score and larger volume than the anesthetic group (n = 11). *P < 0.05; **P < 0.01. An extra experiment was performed with conscious (n = 3) and anesthetic (n = 3) rats going through all the protocol except for MRI scanning (‘No MRI’) showing no significant difference in both NSS and infarct volume compared with the original groups. c Representative TTC-stained brain slices showed larger damaged brain areas (white) in the conscious group after photothrombotic stroke