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Table 1 Composition of normal Tyrode’s solution and the pipette solution used in this study

From: Important modifications by sugammadex, a modified γ-cyclodextrin, of ion currents in differentiated NSC-34 neuronal cells

Solution

Purpose or name

Composition

Bathing solution

Normal Tyrode’s solution

136.5 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl2, 0.53 mM MgCl2, 5.5 mM glucose, and 5.5 mM HEPES–NaOH buffer, pH 7.4

Pipette solution

For recordings of K+ current or membrane potential

130 mM K-aspartate, 20 mM KCl, 1 mM KH2PO4, 1 mM MgCl2, 3 mM Na2ATP, 0.1 mM Na2GTP, 0.1 mM EGTA, and 5 mM HEPES–KOH buffer, pH 7.2

For recordings of Na+ or Ca2+ currents

130 mM Cs-aspartate, 20 mM CsCl, 1 mM KH2PO4, 1 mM MgCl2, 3 mM Na2ATP, 0.1 mM Na2GTP, 0.1 mM EGTA, and 5 mM HEPES–CsOH buffer, pH 7.2