Skip to main content

Table 1 Composition of normal Tyrode’s solution and the pipette solution used in this study

From: Important modifications by sugammadex, a modified γ-cyclodextrin, of ion currents in differentiated NSC-34 neuronal cells

Solution Purpose or name Composition
Bathing solution Normal Tyrode’s solution 136.5 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl2, 0.53 mM MgCl2, 5.5 mM glucose, and 5.5 mM HEPES–NaOH buffer, pH 7.4
Pipette solution For recordings of K+ current or membrane potential 130 mM K-aspartate, 20 mM KCl, 1 mM KH2PO4, 1 mM MgCl2, 3 mM Na2ATP, 0.1 mM Na2GTP, 0.1 mM EGTA, and 5 mM HEPES–KOH buffer, pH 7.2
For recordings of Na+ or Ca2+ currents 130 mM Cs-aspartate, 20 mM CsCl, 1 mM KH2PO4, 1 mM MgCl2, 3 mM Na2ATP, 0.1 mM Na2GTP, 0.1 mM EGTA, and 5 mM HEPES–CsOH buffer, pH 7.2