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Fig. 3 | BMC Neuroscience

Fig. 3

From: Important modifications by sugammadex, a modified γ-cyclodextrin, of ion currents in differentiated NSC-34 neuronal cells

Fig. 3

Evaluation of the kinetics of SGX-induced block of I K(DR). The I K(DR) established by depolarizing pulses from −50 to +50 mV was measured with cell treatment of different SGX concentrations. In A, activation time courses of SGX-sensitive I K(DR)act) obtained during cell exposure to 10 μM (a) and 30 μM (b) SGX were fitted by a single exponential with a value of 27.1 and 15.9 ms respectively. SGX-sensitive current was taken by subtracting current in SGX-treated cells from that in untreated ones. In B, the reciprocal of activation time constant (i.e., 1/τact) of SGX-sensitive current was plotted against the SGX concentration. Data points shown in open circles were well fitted by a linear regression, indicating that there is a molecularity of one. According to reaction scheme, blocking (k +1) and unblocking (k −1) rate constants for SGX-induced blocks of I K(DR) were calculated to be 0.0012 ms−1 μM−1 and 0.021 ms−1 respectively. Mean ± SEM (n = 8–11 for each point)

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