Fig. 2

Effect of SGX on the activation curve of I _K(DR) recorded from differentiated NSC-34 neuronal cells. In these experiments, cells were bathed in Ca²⁺-free Tyrode’s solution. Voltage dependence of I _K(DR) activation in the absence (filled square symbol) and presence (open square symbol) of SGX (30 μM) treatment (mean ± SEM; n = 9 for each point) is illustrated. The smooth lines obtained in untreated and SGX-treated cells represent the best fits to the Boltzmann equation described in “Methods”. Notably, SGX treatment can shift the activation curve of I _K(DR) to a depolarizing voltage, together with increase in the gating charge of this current