Fig. 7

Influence of molar loss and powder diet on BDNF and TrkB mRNA levels in the hippocampus and hypothalamus of 4 and 16 week periods. mRNA levels were determined by RT-PCR and normalized relative to the level of GAPDH mRNA detected in each sample. Two-way ANOVA were used to compare the groups. 4 W Hippocampus BDNF; F(1, 20) = 4.36, P = 0.05 for molar loss, F(1, 20) = 1.19, P = 0.29 for powder diet, F(1, 20) = 1.08, P = 0.31 for molar loss × powder diet. 4 W Hippocampus TrkB; F(1, 20) = 0.48, P = 0.50 for molar loss, F(1, 20) = 0.00, P = 0.99 for powder diet, F(1, 20) = 0.04, P = 0.85 for molar loss × powder diet. 16 W Hippocampus BDNF, F(1, 20) = 46.33, P < 0.01 for molar loss, F(1, 20) = 0.71, P = 0.41 for powder diet, F(1, 20) = 4.52, P < 0.05 for molar loss × powder diet. 16 W Hippocampus Trk B; F(1, 20) = 2.82, P = 0.11 for molar loss, F(1, 20) = 0.18, P = 0.67 for powder diet, F(1, 20) = 17.86, P < 0.01 for molar loss × powder diet. 4 W Hypothalamus BDNF; F(1, 20) = 0.53, P = 0.48 for molar loss, F(1, 20) = 0.14, P = 0.71 for powder diet, F(1, 20) = 0.05, P = 0.82 for molar loss × powder diet. 4 W Hypothalamus TrkB; F(1, 20) = 0.05, P = 0.82 for molar loss, F(1, 20) = 0.00, P = 0.98 for powder diet, F(1, 20) = 0.08, P = 0.78 for molar loss × powder diet. 16 W Hypothalamus BDNF; F(1, 20) = 16.71, P < 0.01 for molar loss, F(1, 20) = 1.13, P = 0.30 for powder diet, F(1, 20) = 0.86, P = 0.36 for molar loss × powder diet. 16 W Hypothalamus TrkB; F(1, 20) = 0.14, P = 0.71 for molar loss, F(1, 20) = 0.33, P = 0.57 for powder diet, F(1, 20) = 0.38, P = 0.54 for molar loss × powder diet. **, *P < 0.01, 0.05 vs respective extracted molar mice at same diet, ^†P < 0.05 vs powder diet at extracted molar mice