Fig. 4

Increase of early endosomes, but not lysosomes, late, or recycling endosomes, in LRRK2-KO microglia. a Immunostaining for lysosomal markers in KO and WT microglia. Lysosomes and acidic lysosomes were visualized using anti-LAMP1 and pHrodo, respectively. b Immunostaining for endosomal markers in KO and WT microglia. Early endosomes, late endosomes, and recycling endosomes were visualized using antibodies against Rab5, Rab7 and Rab11, respectively. Scale bar 20 μm. Quantification of fluorescence intensity per cell showing an increase of Rab5-positive endosomes, but not Rab7- and Rab11-positive particles, in KO microglia. Twenty-five cells were analyzed per group. c Visualization of early endosomes using CellLight. Twenty-five cells were analyzed per group. In the graphical representation, data are expressed as mean ± SD and were assessed by Student’s t test; *p < 0.05. All experiments were carried out three times using primary microglia isolated from independent mice, and a representative image and data are shown