Fig. 2

Increased αSYN uptake and degradation by LRRK2 KO microglia. a Western analysis of LRRK2-KO microglia treated with αSYN. Lysates were prepared from LRRK2- KO and WT microglia treated with or without αSYN and subjected to Western analysis using anti-LRRK2, β-actin, and αSYN (C-20) antibodies. b Quantified density of αSYN bands in (a) normalized by the density of β-actin. n = 4 culture wells per group. c Western analysis using anti-LRRK2, GAPDH, and αSYN (MJFR1) antibodies. d Quantified density of αSYN bands in (c) normalized by the density of GAPDH. n = 4 culture wells per group. e Residual αSYN in the culture media of WT and KO microglia. n = 8 culture wells per group. The amounts of αSYN were measured by ELISA 48 h after αSYN treatment. In all graphical representations, data are expressed as mean ± SD and were assessed by Student’s t test (KO vs. WT); *p < 0.05, **p < 0.01. All experiments were carried out three times using primary microglia isolated from independent mice, and a representative image and data are shown