Skip to main content
Fig. 2 | BMC Neuroscience

Fig. 2

From: Bone morphogenetic protein-2-mediated pain and inflammation in a rat model of posterolateral arthrodesis

Fig. 2

Increased numbers of macrophages in the DRG and spinal nerve of rats implanted with either rhBMP-2/ACS or vehicle/ACS. a, b QPCR analysis of RNA isolated from ipsilateral and contralateral DRGs 1, 3 or 7 days after implantation of rhBMP-2/ACS or vehicle/ACS. There was no induction of S100a8 mRNA (a neutrophil marker) at any time point, but at 7 dps, CD68 mRNA was induced ipsilateral to implantation of either rhBMP-2/ACS or vehicle/ACS (mean ± SEM, n = 5, *p < 0.05). c Representative immunohistochemistry image showing ED1+ cells 8 dps in the DRG and spinal nerve of rhBMP-2 and vehicle ACS implanted rats. d Number of ED1+ macrophages/field in rhBMP-2 or vehicle (control) rats in the DRG (green arrowhead), spinal nerve (the pink arrowhead most proximal to DRG) and ventral rami (remaining arrowheads depicting different locations) (mean ± SEM, n = 6 animals/group). e, f Representative double staining of spinal nerve from 7 dps rhBMP-2/ACS animals with anti-ED1 and anti-IBA1 antibodies. Images show that all ED1+ cells are IBA1+ (yellow arrows). There were, however, IBA1+ cells that were ED1− (white arrow) and these cells were generally smaller than the ED1+ population. Higher magnification (× 40) reveals multiple vacuoles in ED1+ population of macrophages (F)

Back to article page