Table 1 Primers for actb (β-actin), egfp (enhanced green fluorescent protein), rho (rhodopsin), gnat2 (guanine nucleotide-binding protein G protein), pde6h (phosphodiesterase 6H), opn1lw2 (opsin 1 cone pigments long-wave-sensitive) and pde6c (phosphodiesterase 6C) were designed complementary to Expressed Sequence Tags (ESTs) identified after BLAST analysis of EST databases
From: A method for isolation of cone photoreceptors from adult zebrafish retinae
Gene | F/R | Primer sequence | Product size (bp) | Melting temperature (°C) | Annealing temperature (°C) |
|---|---|---|---|---|---|
actb | F | CGAGCAGGAGATGGGAACC | 100 | 61.0 | 53 |
R | CAACGGAAACGCTCATTGC | 58.0 | |||
egfp | F | ATGGTGAGCAAGGGCGAGGAGCTGT | 713 | 68.0 | 63 |
R | TACAGCTCGTCCATGCCGAGAGTGATCC | 70.0 | |||
rho | F | AGAACCATGCCATCATGGGG | 138 | 60.1 | 55 |
R | GAGTGCGGGTGTAGTAGTCG | 59.9 | |||
gnat2 | F | ACGGTCAAACTTCTGCTGCT | 394 | 60.0 | 50 |
R | TGCAGATTCTGTCCATTTCG | 55.0 | |||
pde6h | F | GACCACTCGCACCTTCAAGA | 99 | 59.9 | 55 |
R | ACAGTGATGTCTGTGCCGAG | 60.0 | |||
opn1lw2 | F | TGATGGCTCTGAGGTGTCCA | 105 | 60.5 | 53 |
R | TCCAGTTCTTCCCTCTTGTTCA | 58.9 | |||
pde6c | F | CACAGTTCCTGGGATGGTCC | 116 | 60.0 | 55 |
R | CGGAGTGGCTTTGGTCTGAT | 60.0 |