Fig. 1

Schematic overview of the main steps to purify adult zebrafish cone photoreceptors. 1 Adult Tg(3.2TaCP:EGFP) zebrafish were enucleated using sterilised forceps. 2 Adult zebrafish were enucleated using sterilised forceps stored in DEPC-PBS (a) from the optic nerve (b) and then kept in cold DEPC-PBS. Cornea was pierced (c) and lens removed with a needle (d) to facilitate the onset of the dissection (left picture). EGF-cone photoreceptor retinas (e) were dissected from the eyes (c) and the retinal pigment epithelium (RPE) (f) with sterilised forceps (middle picture). Most of the RPE (f) was removed from the dissected retinas (e) (right picture). 3 Retinal cells were dissociated with trypsin. 4 EGFP-positive cone photoreceptors were purified by flow-cytometry. 5 High-quality RNA was obtained from sorted-GFP-positive cone photoreceptors. 6 Reverse transcriptase-PCR (RT-PCR) validated the purification of cone photoreceptors