Fig. 4

TfAP-2 is required in the adult brain for effective night sleep. A Pan-neuronal knockdown of TfAP-2 in adult neurons reduces night sleep. In order to restrict knockdown of TfAP-2 in neurons only during adulthood a temperature-sensitive Gal80 allele was used. These flies were reared at permissive temperature of 18 °C and then switched to restrictive temperature of 30 °C after adult emergence. The sleep assay was also conducted at 30 °C. UAS-TfAP-2 ^RNAi (v101552) strain was used for knockdown of TfAP-2 in these experiments. B Total sleep, relative day sleep and relative night sleep of the parental controls and mutant flies. C TfAP-2 functions in larval and adult Drosophila brain. *p < 0.05 determined by one way ANOVA, Dunnet post hoc test