Fig. 2

TfAP-2 is expressed in larval and adult Drosophila brain. Antibodies against TfAP-2 were used to reveal the TfAP-2 expression pattern in developing larval (A, B) and adult (C) Drosophila brains. A c41Gal4 driver expressing membrane bound GFP was used to mark contour and the central brain regions. In 3rd instar larvae TfAP-2 was found to have strong expression in the subsets of neurons located in the central brain (yellow arrows), medulla (parenthesis) and lamina (blue arrows) regions. B In larval brain TfAP-2 is also expressed in some of TimGal4 > CD8GFP expressing neurons (arrows). C In adult brain TfAP-2 protein can be found in ventral lateral neurons marked with sNPF > CD8GFP (blue arrows) and anti-PDF staining (yellow arrows). D TfAP-2 expression in l-LNv neurons marked with TimGal4 > CD8GFP and anti-PDF in adult brain. E TfAP-2 expression in l-LNv neurons marked with anti-PDF in TimGal4 > TfAP-2RNAi mutant adult brain. F Bar graph shows relative intensity of TfAP-2 antibody staining in PDF-expressing neurons for TimGal4 > CD8GFP (control) and TimGal4 > TfAP-2RNAi animals. Antibody staining intensity (Mean ± Average deviation) is 1 ± 0.33 (n = 27) for control, and 0.51 ± 0.17 (n = 10) for TfAP-2RNAi. n, number of l-LNv neuronal clusters. Statistical significance of TfAP-2 downregulation was calculated by Student t test, p = 0.00023