Skip to main content
Fig. 1 | BMC Neuroscience

Fig. 1

From: TfAP-2 is required for night sleep in Drosophila

Fig. 1

Neuronal TfAP-2 is required for night sleep. A TfAP-2 is a well-conserved transcription factor present in most multicellular organisms of the animal kingdom. The multiple alignment and phylogenetic tree were computed with Clustal W phylogeny [25]. B The sleep profile of adult flies was generated using a video monitoring protocol and extracted by PySolo. Expression of Dcr-2 and TfAP-2 RNAi were induced in neurons throughout the life history of these flies. C Quantification of sleep in Dcr-2 and TfAP-2 RNAi flied. D Sleep profile of flies was recorded using Trikinetics infra-red beam crossing and processed by a counting macro. Neuronal knockdown of TfAP-2 results in drastic reduction of night sleep in the presence of Dcr-2. The phenotype is weaker when knockdown is achieved without exogenous Dcr-2. UAS-TfAP-2 RNAi (v101552) strain was used for knockdown of TfAP-2 in these experiments. E Quantification of sleep parameters measured in d. ***p < 0.0001 Student t test. *p < 0.05 determined by one way ANOVA, Dunnet post hoc test

Back to article page