Fig. 4
From: Severe respiratory changes at end stage in a FUS-induced disease state in adult rats
FUS and GFP transgene expression. a Transfection of the FUS DNA construct resulted in FUS overexpression in HEK293T cells at the expected molecular weight. b AAV9 GFP expression in the lumbar spinal cord of a rat that received AAV9 GFP 8 weeks earlier. GFP immunofluorescent staining is in b, SMI 311, a motor neuron marker antibody immunofluorescence is in c, and the merger is in d. The co-localization indicates GFP expression in motor neurons. Bar in d is 134 μm