Fig. 1

FUS gene transfer causes progressive motor impairments. A There was an indication of lowered weight gain in FUS rats by ANOVA (see “Results”). The FUS group weighed significantly less than the vehicle and GFP groups at 3 and 4 weeks post-injection (p < 0.05–0.01). B FUS rats were significantly impaired for rotarod performance by ANOVA with significant differences found at 1, 3, or 4 weeks compared to the vehicle group (p < 0.05–0.001) and at 4 weeks compared to the GFP group (p < 0.01). C All of the FUS rats developed hindlimb clenching during the escape reflex while no vehicle or GFP rats showed abnormal hindlimb clenching during the study (p < 0.01). D The FUS group had decreased forelimb strength at 4 weeks compared to both vehicle and GFP (p < 0.001). There were no significant differences between the vehicle and GFP groups in any of these measures. “a”, significant difference between FUS and vehicle groups; “b”, significant difference between FUS and GFP (ANOVA/Bonferroni post-tests in A, B, D and Mantel-Cox log rank analysis in C; n = 4–6/group as indicated)