Fig. 1From: Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafishSchematics outlining the experimental design. The larval zebrafish were treated with various acute stressors (Heat stimulus, cold stimulus, UV light, social isolation and mechanical disturbance) for 5 min. After 5-min stressor treatment, larval zebrafish were transferred from the dish to the light/dark test apparatus for light/dark preference behavioral assay and recorded for 8 min, or collected with ice-cold phosphate buffered saline (PBS) and transferred to collection tubes on dry ice for cortisol assayBack to article page