Fig. 2From: The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junctionQuantification of Dlg width. a Anti-Dlg was used to immuno-label the SSR and images were analyzed with ImageJ software. An octagonal overlay was placed on the image and a line tool was used to quantify the average length of eight spokes, producing the average width of Dlg fluorescence for a single bouton type. b The quantification of Dlg width for each genotype, where each bar represents the mean ± SEM. n = 15 boutons for each genotype (One-way ANOVA Bonferroni post test, not significant n.s., **p < 0.001; ***p < 0.0001)Back to article page