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Table 1 siRNA sequences used to knock down protein expression

From: Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer’s disease

siRNA target Sequence References
Total PICALM ‘A’a GCAUACAAUGAAGGAAUUAdTdT Custom-designed
Total PICALM ‘B’a AAGCCAAGAAUUGCUAUGAdTdT Custom-designed
PICALM isoform 1 GCAAGUACAUGGGGAGAUCdTdT Custom-designed
PICALM isoform 2 GUACAUGGGGAGGAUUCACdTdT Custom-designed
CHC UAAUCCAAUUCGAAGACCAAUdTdT [43]
GFP GGCUACGUCCAGGAGCGCACCdTdT [43]
  1. aThe siRNA sequences for total PICALM were directed against regions of the mRNA common to isoforms 1–4
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