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Fig. 9 | BMC Neuroscience

Fig. 9

From: Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer’s disease

Fig. 9

Differential effects of clathrin siRNA on isoforms 1 and 2 of PICALM and APP processing. H4 cells were incubated with media, control siRNA (GFP siRNA) or siRNA targeting clathrin heavy chain (Clathrin siRNA) for 48 h. a Representative immunoblot of isoforms 1 and 2 of PICALM and b following densitometric analysis of isoform 1 and c isoform 2. Data are presented as a percentage of the untreated media control after normalisation to house-keeping protein levels and are mean ± SEM. Levels of d APP, e βCTF and f BACE1 as detected by ELISA. Data are expressed as % of media control and are mean ± SEM. N = 3 for all, *p < 0.05 significantly different to GFP siRNA with an unpaired Student’s t test

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