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Fig. 8 | BMC Neuroscience

Fig. 8

From: Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer’s disease

Fig. 8

Effects of clathrin siRNA on clathrin-mediated endocytosis. H4 cells were incubated with media, control siRNA (GFP siRNA) or siRNA targeting clathrin heavy chain (Clathrin siRNA) for 48 h. a Representative immunoblot of clathrin expression and b following densitometric analysis. Data are presented as a percentage of the untreated media control after normalisation to house-keeping protein levels and are mean ± SEM, n = 3, *p < 0.05 significantly different to GFP siRNA with an unpaired Student’s t test. c Clathrin mRNA expression levels using quantitative PCR, normalised to the housekeeping gene 18S and relative to the untreated cells. Data are presented as mean ± SEM, n = 6, **p < 0.01 significantly different to GFP siRNA with Mann–Whitney-U. d H4 cells were incubated with 100 nM Alexa488-Tf for 5, 15 or 30, minutes then trypsinised and analysed by flow cytometry. Mean fluorescent intensity (MFI) data are expressed as fold change over untreated control cells (0 min Alexa488-Tf) and are mean ± SEM *p < 0.05,**p < 0.01 significantly different to GFP siRNA with an unpaired Student’s t test, n = 4–5

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