Fig. 6

PICALM levels following siRNA treatments targeting isoforms 1 and 2 of PICALM, total PICALM and clathrin. a Representative immunoblots of PICALM isoforms after incubating H4 cells for 48 h with media or siRNA targeting either total PICALM (sequence A), PICALM isoform 1, PICALM isoform 2, clathrin or GFP for 48 h. b Densitometric Western blot analysis of the levels of PICALM, isoform 1 and c isoform 2. Data are presented as a percentage of the untreated media control after normalisation to house-keeping protein levels and are mean ± SEM, n = 6–7, *p < 0.05, ***p < 0.001 significantly different to GFP siRNA following one-way analysis of variance and Dunnett’s multiple comparison test (b) or Kruskal–Wallis test and Dunn’s multiple comparison test (c). d PICALM isoform 1 and e PICALM isoform 2 mRNA expression levels measured using quantitative PCR, normalised to the housekeeping gene POLR2A and relative to the untreated cells, n = 5–6, ***p < 0.001 significantly different to GFP siRNA following one-way analysis of variance and Dunnett’s multiple comparison test