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Fig. 5 | BMC Neuroscience

Fig. 5

From: Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer’s disease

Fig. 5

Effects of PICALM and clathrin siRNA on APP processing. H4 cells were incubated with media, control siRNA (GFP siRNA), siRNA targeting PICALM (PICALM siRNA sequence A or B) or clathrin heavy chain (Clathrin siRNA) for 48 h. Levels of APP after treatment with (a, PICALM siRNA Seq A) and (b, PICALM siRNA Seq B), c Aβ40, d sAPPβ:sAPPα, e βCTF and h BACE1 as detected by ELISA. Data are expressed as % of media control (ac, e, h) and are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 significantly different to GFP siRNA with an unpaired Student’s t test, n = 11 (a), n = 4 (b), n = 5 (c) n = 4 (d), n = 7 (e), n = 4 (h). f APP and g BACE1 mRNA expression levels measured using quantitative PCR, normalised to the housekeeping gene POLR2A and relative to the untreated cells. Data are expressed as mean ± SEM, *p < 0.05 significantly different to GFP siRNA with one-way analysis of variance and Dunnett’s multiple comparison test n = 6. Unless specified, cells were treated with siRNA targeting PICALM sequence A

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