Fig. 2
From: TNFAIP1 contributes to the neurotoxicity induced by Aβ25–35 in Neuro2a cells
Effect of TNFAIP1 on Aβ25–35-induced neuronal cell death. a TNFAIP1 protein levels were measured by Western blot in N2a cells cultured in 12 wells plate and transfected with 20 pmol of Control siRNA or TNFAIP1 siRNA. b MTT reduction was measured by MTT assay in N2a cells cultured in 12 wells plate and transfected with 20 pmol of Control siRNA or TNFAIP1 siRNA, followed by treated by dose-dependent Aβ25–35 (0–20 μM). Data are expressed as mean ± SD, n = 4, *p < 0.05 compared with Aβ25–35 (0 μM), #p < 0.05 compared with Control siRNA plus Aβ25–35 (20 μM). c Myc-TNFAIP1 protein levels were measured by Western blot in N2a cells cultured in 12 wells plate and transfected with 1.2 μg of pCMV-Myc or Myc-TNFAIP1. d MTT reduction was measured by MTT assay in N2a cells cultured in 12 wells plate and transfected with dose-dependent Myc-TNFAIP1 (0–1.2 μg), followed by treated by PBS or Aβ25–35 (20 μM). Data are expressed as mean ± SD, n = 4, *p < 0.05 compared with Myc-TNFAIP1 (0 μg) plus PBS. #p < 0.05 compared with Myc-TNFAIP1 plus PBS