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Fig. 7 | BMC Neuroscience

Fig. 7

From: The preventive effect of resiniferatoxin on the development of cold hypersensitivity induced by spinal nerve ligation: involvement of TRPM8

Fig. 7

Immunofluorescence view of transient receptor potential mellastatin type 8 (TRPM8) expression after resiniferatoxin (RTX) pretreatment. Perineural administration with vehicle or RTX (0 or 1 µg) was performed before spinal nerve ligation (SNL). To evaluate the protein expression level of TRPM8 located in the DRG, the ipsilateral (left) L5 and L6 DRG were isolated 4 weeks after SNL and confocal immunofluorescence analysis was performed. Mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI; blue) counterstain was dispersed over the sections before mounting. a TRPM8 expression (red; arrows) in the DRG of sham-operated control group rats. b TRPM8 expression (red; arrows) in the DRG of RTX 0 µg group rats. TRPM8 expression in the DRG was up-regulated and the co-localization of TRPM8 expressing neurons with NF200-positive neurons (green) was increased (arrow heads). c TRPM8 expression (red; arrows) in the DRG of RTX 1 µg group rats. TRPM8 expression was suppressed compared to that of the RTX 0 µg group

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BMC Neuroscience

ISSN: 1471-2202

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