Fig. 6

The time-dependent mGDNF level in the striatum after HEK/mGDNF/GFP transplantation. a–c Representative fluorescence micrographs of coronal sections of the striatum in the transplantation site. a GFP-expressing cells were almost missing in the injection site (indicated by the arrow) in the caudate-putamen of animals sacrificed 19 days after transplantation of transgenic cells containing the modified GDNF. One can see two GFP-expressing cells. b mGDNF/GFP-producing cells 3 days after transplantation into the striatum of mouse brain. c The same section immunohistochemically stained for GDNF (visualized by Texas red-conjugated secondary antibodies). Scale in a 1 mm, in b, c (shown on c) 200 μm. d The results of Western blot analysis of mGDNF/GFP expression in striatum 2, 3, 5, 18 days after cell administration