Fig. 2

Interneuron migration is altered, but progenitor domains are not at e14.5. For all panels—upper row is control sections, lower row is male CKO. A Re-presenting A from Fig. 1 for reference and highlighting anatomy of ventral foerbrain. B In situ hybridization for Gad67 demonstrates normal appearance of cells in the ventral pallium (asterisk) and substantial decrease in migrating cells (arrows) in the cortex with remaining cells migrating in the SVZ (arrows). C–C′ Further confirmation of preservation of ventral interneuron progenitor pool with Dlx5/6—tdTomato fluorescence being unchanged between wildtype and mutant embryos. Anatomy of section is highlighted by dashed white outline. D–D′ Co-labelling of Dlx5/6-GFP in green, Nxk2.1 in red and nuclei in blue (DAPI labeling) shows normal Nxk2.1 expression and normal Dlx5/6 expression, again with reduction of neurons migrating to cortex. Again note with DAPI labeling that anatomy of ventral forebrain is unchanged in the male CKO. LV lateral ventricle, SVZ subventricular zone, VZ ventricular zone, BG basal ganglia, GE ganglionic eminence, Cx developing cortex