Fig. 6

SAHA inhibited TLR4 gene transcription by histone acetylation regulation. Histones interaction with the 5′ region of the TLR4 gene were examined with ChIP assays using anti-acetyl histone a H3 and b H3K9 antibodies. Rabbit IgG was used as a negative control. The “input” lanes represent the results of PCR using non-immunoprecipitated chromatin as templates. The 5′ regions of the TLR4 gene, with the 5′ regions of the GAPDH gene as an internal reference, were amplified by PCR from the immunoprecipitated chromatin. The DNA bands for c H3 and d H3K9 were quantified by densitometry and data expressed as mean ± SD (n = 3). Bars expressed as the ratio of optical density (OD) of the samples to the corresponding internal standard (GAPDH). *P < 0.05; **P < 0.01; ***P < 0.001; ^#P > 0.05