Fig. 2

SAHA attenuated KA-induced neuronal death in hippocampal CA1 region. SAHA (10 or 50 mg/kg) was administrated intraperitoneally 30 min before KA injection. Neuronal apoptosis were evaluated 3 days after KA injection. (a–d) TUNEL staining of hippocampal CA1 pyramidal neurons; a control group, b KA group, c KA + 10 mg/kg SAHA group, d KA + 50 mg/kg SAHA group, e TUNEL positive cells quantities for each group. Data are expressed as mean ± SD. Scale bar = 100 μm. *P < 0.05, KA group versus KA + 10 mg/kg SAHA group; **P < 0.01, KA group versus KA + 50 mg/kg SAHA group; ***P < 0.001, KA group versus control group; ^#P < 0.001, KA + 10 mg/kgSAHA group versus KA + 50 mg/kg SAHA group