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Fig. 5 | BMC Neuroscience

Fig. 5

From: A non-aggressive, highly efficient, enzymatic method for dissociation of human brain-tumors and brain-tissues to viable single-cells

Fig. 5

Comparison between trypan-blue and flow-cytometry to determine cellular viability. a Cells dissociated from a GBM sample, stained with ViViD, an amine-reactive viability dye, and flow cytometrically analyzed (b) photographs of dissociated (c) brain cells and (d) BT cells, stained using trypan blue. d Correlation between percent viability determined by trypan blue (mean = 77 %) and flow cytometry (mean = 75 %), sixteen samples depicted

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