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Fig. 2 | BMC Neuroscience

Fig. 2

From: A non-aggressive, highly efficient, enzymatic method for dissociation of human brain-tumors and brain-tissues to viable single-cells

Fig. 2

BT dissociation to single cells using dispase (Disp) or neutral protease (NP). a Cellular viability and b dissociation quality (CG) of BTs dissociated with dispase or NP, at the respective enzyme’s optimal dissociation time. Following indicated times (1 or 2 h) the cells were triturated using a Pasteur pipette and their viability and CG were determined. Oli—Oligodenderoglioma, OliAst–Oligoastrocytoma, Ast–Astrocytoma, GBM–Glioblastoma, Mets–Metastasis to the brain (lung–lun and melanoma–Mel), Epi + Br—Epileptic foci, and peritumoral brain tissue. Parenthesis indicate the grade of the tumors, e.g. Oli(2–3). Statistics Viability following dissociation of all glial tumors (Oli, OliAst, Ast, GBM) using NP-2 h to dispase-1 h (P < 0.01)

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