Fig. 4
3-NT immunoreactivity of spinal cord slice cultures following treatment with light of varying wavelengths and intensities. A–D Quantification of the area of 3-NT immunoreactivity above a set threshold in spinal cord slice cultures treated with 450, 510, 660 or 860 nm light. Low (1.93 × 1016), intermediate (3.85 × 1016), or high (7.70 × 1016) (photons/cm2/s) intensities were delivered. Significant differences from control for each intensity of light delivered are indicated by *(p ≤ 0.05). E–Q Representative images are provided for each treatment intensity and wavelength, imaged immediately adjacent to the cut: 3-nitrotyrosine immunoreactivity (3NT) is red, together with β-III tubulin immunoreactivity to identify axons (green) and Hoechst nuclear stain (blue) for control (E), 450 nm treated (F–H), 510 nm treated (I–K), 660 nm treated (l–N) or 860 nm treated (O–Q) cultures. Scale = 50 µm for all images. Each experiment was conducted three times and data from a single experiment with 8 replicates per group are shown