Fig. 3

The effects of cut injury on spinal cord slice cultures from neonatal mouse. Spinal cord slice cultures were cultured for 10 DIV, and either not cut (A–D), or subjected to cut injury (E–H). Immunohistochemical analysis of NFH (red), MBP (green) and MnSOD (blue) with overlay in uncut and cut cultures are shown at 11 DIV (A–H), scale = 10 µm. Immunohistochemical analysis of NFH (red), MBP (green) with overlay (I–K) and overlay with Hoechst (L) in cut cultures are shown at 17 DIV (I–L), scale = 20 µm