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Fig. 3 | BMC Neuroscience

Fig. 3

From: Metabolomic investigation of regional brain tissue dysfunctions induced by global cerebral ischemia

Fig. 3

The OPLS-DA score plots and loading plots derived from the 600 MHz 1H NMR spectra of cortex samples (a, a′) and hippocampus samples (b, b′) extracted from the mice in male sham group (filled square) and male experimental group (triangle). The male hippocampus sham group contains five samples (n = 5), and the other three groups contain six samples (n = 6) in each. The values of Q2 parameter, which were >0.4, indicated that the established OPLS-DA models were valid. Those metabolites with the value of variable importance in the projection (VIP) bigger than 1 and the absolute value of correlation coefficient |r| greater than the cutoff value 0.576 (the sum of two group samples equals to 12, n = 12) or 0.602 (the sum of two group samples equals to 11, n = 11) were defined to be the discriminative metabolites for group clustering. The discriminative metabolites with the value of correlation coefficient r > 0.576 (n = 12) or r > 0.602 (n = 11) indicated that their levels increased upon the global ischemic attack. And those discriminative metabolites with the value of correlation coefficient r < −0.576 (n = 12) or r < −0.602 (n = 11) indicated that their levels decreased upon the global ischemic attack

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