Fig. 5

Analysis of the cortical myelination screen of the NCC compound library. a High-throughput screening data set used to identify promoters of myelination. The mean response is indicated by the solid line. The dotted line delineates the value of three SDs above the mean. Compounds that significantly reduced Olig2 expression were excluded. b High-throughput screening data plate control values of myelination. Each point is an compiled control value from each screening plate (n = 44, 16 image fields per concentration, mean ± SEM *P < 0.0001, t test). c Using the fiber score/MBP score as a specific measure of myelination (see Additional file 10: Fig. S10), the ratio of the DAPT to DMSO controls demonstrates the screening assay window. The red line delineates the cutoff value of 1.3. Each point is an averaged value from each screening plate (32 image fields per condition, mean ± SEM). The average DAPT/DMSO-fiber score/MBP score for the entire NCC library screen = 1.61 (dashed line). d NCC library hit selection process in the cortical culture myelination assay. Fifty-three primary hits compounds were initially identified from the NCC library with the criteria of >50 % DAPT and >1.5 fiber score/MBP score or fiber/MBP. The primary hits were further refined with additional criteria of >25 % DAPT/Olig2 nuclei ratio, <40 % DAPI/Olig2 nuclei ratio, and a visual morphology check to yield refined hits of 33 compounds. All refined hit compounds were reordered fresh and tested for efficacy in a dose–response profile. Ten compounds passed these criteria and were confirmed as hits