Fig. 3
From: Development of a central nervous system axonal myelination assay for high throughput screening
Half maximal effective concentration determination of four different GSIs for the promotion of myelination in the cortical culture assay. Dose response data confirm the activity of GSIs and enable the calculation of the EC50 value for each compound. Cortical cultures were treated for 8 days with DAPT, LY 411,575, BMS 708,163 or MRK 560 and immunostained for MBP, Olig2 and DAPI. Dose–response curve for DAPT is compiled from n = 3 experiments, 80 image fields per concentration. Representative dose–response curves for LY 411,575, BMS 708,163 and MRK 560 are 32 image fields per concentration, mean ± SEM. Respective EC50 values are shown in the legend