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Fig. 1 | BMC Neuroscience

Fig. 1

From: Development of a central nervous system axonal myelination assay for high throughput screening

Fig. 1

Flow scheme for the cortical cell myelination assay. a Dissociated cells from the cortex containing neurons and glial progenitor cells were cultured from E18 rat embryos onto poly-d-lysine/laminin coated 96-well plates. b On DIV4, when axonal projections (red) are apparent in the neuronal population, the growing co-culture was changed to MyM media to induce OL differentiation and initiate myelination. The following day test compounds were added and cultures were left undisturbed for an additional 8 days. c Cells were fixed and immunostained for MBP, Olig2 and DAPI on DIV13. Images were acquired using automated microscopy and scored phenotypically for myelination as described in the “Methods

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