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Fig. 3 | BMC Neuroscience

Fig. 3

From: RETRACTED ARTICLE: miR-320a affects spinal cord edema through negatively regulating aquaporin-1 of blood–spinal cord barrier during bimodal stage after ischemia reperfusion injury in rats

Fig. 3

Screening of miRNAs targeting AQP1 mRNA 3′UTR after IR. A Hierarchical cluster analyses of altered microRNAs (miRs) in spinal cords at 12 h (a) and 48 h (b) after IR (n = 3/group). Each row represents an miR, and each column represents a sample. The miRs that were upregulated are shown in green to red, whereas the miRs that were downregulated are shown from red to green. Among all the significantly changed miRs, miR-320a was reported to be differentially expressed at both 12 and 48 h after IR. B Quantitative real-time polymerase chain reaction (qRT-PCR) analysis confirming the miR-320a was abnormally expressed at both 12 and 48 h after IR. Relative expression is the change in expression compared to the Sham-operated group. Data are expressed as mean ± SEM. C Direct interaction between miR-320a and the 3′-UTR of AQP1. HEK-293T cells were co-transfected with anti- or pre-miR-320a and a pmiR-RB-REPORT™ luciferase reporter plasmid containing with either wild or mutant miR-320a binding site of AQP1. Compared to control-transfected cells, anti-miR-320a exhibited an increase in the relative luciferase expression, whereas pre-miR-320a strongly inhibited luciferase activity. Data are expressed as mean ± SEM. **P < 0.05 versus the control miR. Results shown are representative data from three separate experiments

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