Fig. 5

Transient impairment of OPC migration on laminin in response to ILK loss. a Representative confocal images of Ilk ^−/− and Ilk ^fl/fl OPCAs migrated on Ln for 4, 10 and 24 h. b Quantification of OPC migration using the concentric ring method (see “Methods” section). At the 4 h time point, Ilk ^−/− OPCs accumulated in the most proximal concentric ring (50 μm) and fewer attained the more distant 100 μm ring as compared to Ilk ^fl/fl control cells. Interestingly however, Ilk ^−/− and Ilk ^fl/fl OPCs migrated to the same extent at the 10 and 24 h time points. c No significant difference between the total number of migrated Ilk ^−/− and Ilk ^fl/fl OPCs was found at any time point. d There was no significant difference in initial diameter of Ilk ^−/− and Ilk ^fl/fl OPCAs. Data represent the mean ± SEM (n = 5, 5 and 4 for the 4, 10 and 24 h time points, respectively). For b, *p < 0.05; ****p < 0.0001 (repeated measures two-way ANOVA followed by Bonferroni post-tests). For c and d, data was tested with Student’s t test. Scale bars, 50, 100 and 200 μm for 4, 10 and 24 h time points, respectively